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Behavioral Core Director: Dr. Maria Gulinello The Behavioral Core provides consultation, assistance and behavioral testing equipment for mice and rats. The Behavioral Core aims to facilitate functional testing (cognitive, sensorimotor, emotional and social tasks) at every level while also providing assistance with the design, analysis and publication of behavioral studies (statistics and manuscript writing), the maintenance of animal protocols and training for students, faculty and staff.
Image Analysis and Graphics CoreDirector: Lucy L. Brown, Ph.D. Associate: Kevin Fisher Systems Analyst: Maurice Volaski The Image Analysis and Graphics Core (IAG) at the Kennedy Center provides technical, consultative, and equipment support for the display, manipulation, quantification, transformation and storage of graphic images. The IAG activities include one-on-one tutoring, carrying out analyses, research on software capabilities and the management of computer hardware/software for MRRC investigators. In addition, the IAG supports and provides the use of sophisticated graphics software essential to the preparation of peer-reviewed publications, review chapters, grants and oral presentations. Finally, the IAG supports publication quality printing of images and posters. Images generated in the IAG have appeared on the covers of respected research journals (e.g., The Journal of Neuroscience). As new techniques, such as fMRI and confocal microscopy emerge, the accurate and detailed analysis of graphic images has become a critical tool for neuroscientists and developmental biologists. Image Analysis and Graphics Core Main Page
Molecular and Neurogenomics CoreDirector: R. Suzanne Zukin, Ph.D. Associates: Anna Francesconi, Ph.D Dumitru A. Iacobas, Ph.D. Research Technician: Ms. Adrianna Barrantes The Molecular & Neurogenomics Core (MNC) at the Kennedy Center provides consultation on and assistance with novel molecular, cellular and genetic technologies that facilitate the research objectives of Kennedy Center Investigators. These technologies include in situ hybridization, design and synthesis of customized oligonucleotides, quantitative real-time PCR, site-directed mutagenesis, viral-based gene delivery, protein transduction, laser-capture microdissection, genomics (hybridization, genomic and epi-genomic microarray, genomic database applications) and annotated libraries of small interfering RNAs (siRNAs) for genome-wide gene silencing. The resources and services offered by the MNC support studies relevant to brain development, mental retardation and genome-wide studies of gene organization (and gene delivery), as specified in the MRDD Statement of Mission. Molecular and Neurogenomics Core Main Page
Director: Steven U. Walkley, D.V.M., Ph.D. Associate Director: David Hall, Ph.D. Associate Director: Kostantin Dobrenis, Ph.D. The Morphology Core at the Kennedy Center provides technical, consultative, and equipment support for light, fluorescence, confocal and electron microscopic studies. The Morphology Core consists of three components providing light microscopy and digital photomicroscopy (tissue acquisition and related histological analysis, including animal perfusions, vibratome and freezing microtome sectioning, tissue staining (e.g., immunocytochemistry, Golgi staining, routine staining) and photomicroscopy and camera lucida), ultrastructural analysis (electron microscopic study of tissues) and confocal imaging (optical sectioning capability).Morphology Core Main Page Director: Mark F. Mehler, M.D. Associates: Solen Gohkan, M.D. Peter Mabie, M.D. Guangyu Li, Ph.D. Research Technician: Esther Flaschner Berko The Tissue Culture Core at the Kennedy Center provides technical, consultative and equipment support in standard and advanced neural tissue culture techniques including dissociated cell cultures, organotypic cultures, establishment of new cell lines by conditional-immortalization of neural stem and progenitor cells, establishment of human and animal cell lines from tissue samples, passage and lineage analysis of human and animal cell lines, establishment of single cell and associated clonal density cultures, immunoselection of specific neural stem and progenitor and more mature neuronal and glial progeny, and techniques and associated protocols for the preparation of cultured cells for morphologic, electrophysiologic, biochemical, and molecular biologic analyses. |
